Detection of airborne inoculum of Leptosphaeria maculans and Pyrenopeziza brassicae in oilseed rape crops by polymerase chain reaction (PCR) assays
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چکیده
منابع مشابه
Determining frequencies of avirulent alleles in airborne inoculum of Leptosphaeria maculans using molecular assays
Control of blackleg (phoma stem canker) disease relies on crop management, fungicides and breeding for disease resistance. Proteins encoded by these resistance genes recognize avirulence (Avr) gene products in the fungus during invasion of the plant. However, these Avr genes can mutate resulting in fungal isolates becoming virulent, thus avoiding recognition by the plant. Widespread use of cult...
متن کاملsensitive detection of giardia cysts by polymerase chain reaction (pcr)
giardia is one of the most common human parasites and causes a lengthly course of nonbacterial diarrhea. disease outbreaks due to giardia infection are often attributed to contaminated water supplies. a major problem associated with detection for this organism is the lack of sensitive and reliable methods. pcr has the potential to address many of the limitations.we have performed a pcr-based me...
متن کاملEffects of Leptosphaeria maculans race structure and fitness cost of virulence on management of phoma stem canker in oilseed rape
Fitness of virulent (avrLm1 and avrLm4) or avirulent (AvrLm1 and AvrLm4) isolates of Leptosphaeria maculans on Brassica napus without the corresponding resistance genes Rlm1 and Rlm4 was investigated in controlled environment (CE) and field experiments. Results indicate that there is a measurable fitness cost for virulent isolates compared to avirulent isolates in terms of number of lesions, si...
متن کاملRapid Detection of Campylobacter jejuni by Polymerase Chain Reaction and Evaluation of its Sensitivity and Specificity
Introduction: Campylobacter jejuni is one of the most common causes of food poising in humans. Rapid and specific detection of these bacteria has an important role in diagnosis and treatment of infection. The aim of this study was to design a specific PCR for the detection of Campylobacter jejuni. Methods: In this experimental study, oxidoreductase gene from the Campylobacter jejuni was sele...
متن کاملAssessing Quantitative Resistance against Leptosphaeria maculans (Phoma Stem Canker) in Brassica napus (Oilseed Rape) in Young Plants
Quantitative resistance against Leptosphaeria maculans in Brassica napus is difficult to assess in young plants due to the long period of symptomless growth of the pathogen from the appearance of leaf lesions to the appearance of canker symptoms on the stem. By using doubled haploid (DH) lines A30 (susceptible) and C119 (with quantitative resistance), quantitative resistance against L. maculans...
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ژورنال
عنوان ژورنال: Plant Pathology
سال: 2002
ISSN: 0032-0862
DOI: 10.1046/j.1365-3059.2002.00721.x